EKSPLORASI BAKTERI TERMOHALOFILIK POTENSIAL PENGHASIL L-ASPARAGINASE SEBAGAI ANTIKANKER DI SUMBER AIR PANAS WAWOLESEA

Jamaluddin Jamaluddin, Alfin Alfin, Muzuni Muzuni, Nur Arfa Yanti

Abstract


ABSTRAK

 

Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea. Bakteri termohalofilik penghasil L-asparaginase asal sumber air panas Wawolesea diperoleh dengan tahapan: survey dan pengukuran parameter lingkungan sumber air panas Wawolesea; isolasi bakteri pada media NA (Nutrient Agar) dan seleksi bakteri penghasil enzim L-asparaginase pada media M-9. Hasil isolasi menunjukkan adanya 52 isolat bakteri termohalofilik dan 14 isolat diantaranya mampu menghasilkan L-asparaginase.

 

Kata Kunci: Bakteri Termohalofilik, L-Asparaginase, Sumber Air Panas Wawolesea

 

ABSTRACT


 

The objective of this study was to obtain isolates of thermohalophilic bacteria producing L-asparaginase enzyme from Wawolesea hot spring. L-asparaginase producing thermohalophilic bacteria from the Wawolesea hot spring are obtained by steps; survey and environmental parameters measurement of Wawolesea hot spring, isolation of bacteria on NA (Nutrient agar) medium and selection of L-asparaginase producing bacteria on M-9 medium. The isolation results showed 52 isolates of thermohalophilic bacteria and 14 isolates of them capable of producing L-asparaginase.

 

Keywords: Thermohalophilic bacteria, L-asparaginase, Wawolesea hot spring

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DOI: http://dx.doi.org/10.33772/biowallacea.v5i1.4591

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BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research),

p-ISSN : 2355-6404  l   e-ISSN : 2685-6360

Alamat : Jalan H.E.A. Mokodompit Kampus Baru Anduonohu, Lantai-2 Jurusan Biologi Program Studi Biologi Fakultas MIPA Universitas Halu Oleo, Kendari.

Editor-in-Chief: Mr. Jamili (HP/WA: +6285241767959)

Journal Manager of BioWallacea: L.O.A.P. Rudia (HP/WA: +6285396691601)

E-mail: biowallacea@uho.ac.id

 

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