Daisy D.S.J. Tambajong, B Tulung, J.F Umboh, A Pendong


The genomic DNA extracted from dried blood of B. celebensis was amplified by using primer pair of DTF2
and DTR2 produced a single DNA fragment of 1,140 bp as expected. Sequencing of this fragment confirmed
that the fragment was a fragment of mitochondrial DNA of Cytochrome b gene (Cyt b). Total fragment size
of complete Cyt b gene of B. celebensis is 1,041 bp with no insertions or deletions (indels) in the sequence
and the translated proteins are 379 amino acids. Nucleotide sequence of Cyt b gene of B. celebensis was
aligned with 15 Cyt b genes (Suidae) deposited in GenBank to contruct phylogenetic tree. The genetic
analysis showed three main clusters, one cluster comprised genus Sus, genus Phacochoerus and another
cluster comprised genus Babyrousa with an outgroup of Bubalus depressicornis that is clearly separated.
The sequence similarity (Identity) between B. celebensis and B. babyrussa ranged from 95.2% to 96.4%.
Complete sequence of mitochondrial Cytochrome b gene proved to be practical for identification of a
Babyrousa species unambiguously.

Keywords:  DNA extraction, cloning, sequencing, Babyrousa celebensis, Cytochromeb gene and
Phylogenetic tree

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