Filogeni Beberapa Sapi Lokal Indonesia Menggunakan DNA Mitokondria COI (Cytochrome Oxidase Sub unit I)

Anisa Wulandari, V.M. Ani Nurgiartiningsih, Kuswati Kuswati, Tri Eko Susilorini, Paskah Partogi Agung

Abstract


ABSTRAK

Tujuan penelitian ini adalah untuk mendapatkan konstruksi pohon filogeni berdasarkan keragaman DNA Mitokondria khususnya COI (Cytochrome Oxidase Sub unit I). Sampel yang digunakan yakni darah sapi PO, DNA sapi SO, sapi pesisir, sapi bali, sapi madura dan sapi pasundan masing-masing 12 sampel. Sekuen COI dianalisis menggunakan MEGA 7.0 software dengan metode bootstrapped Neighboor-Joining (NJ) 1000 kali pengulangan berdasarkan between group untuk merekonstruksi pohon filogeni. Hasil penelitian menunjukkan sapi pesisir dekat dengan sapi SO, sapi madura dan sapi pasundan tidak memiliki kekerabatan dengan sapi lokal Indonesia lainnya sehingga membentuk garis tersendiri dan sapi PO dekat dengan sapi bali.

Kata Kunci: COI, DNA mitokondria, filogeni sapi lokal indonesia

ABSTRACT

The purpose of this study was to obtain the construction of phylogeny trees based on the diversity of Mitochondrial DNA especially COI. The samples used were PO cattle blood, SO cow DNA, Coastal cattle, bali cattle, madura cattle, and pasundan cattle every 12 samples. COI sequences were analyzed using MEGA 7.0 software with the bootstrapped Neighbor-Joining (NJ) method 1000 repetitions based on the between groups to reconstruct the phylogeny tree. The results showed that boastal cattle close to SO cattle, madura cattle, and pasundan cattle did not have a kinship with other local Indonesian cows to form a separate line and PO cattle close to bali cattle.

Keywords: mitochondrial DNA, coi, indonesian local cow, phylogeny


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References


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DOI: http://dx.doi.org/10.33772/jitro.v6i2.6165

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