Kinetika dan Karakterisasi Amiloglukosidase Isolat Aaspergillus niger Dari Limbah Ampas Sagu

Amiruddin -

Abstract


Study of characterization and kinetic amyloglucosidase from Aspergillusniger isolate
using sago pith waste has been done. The aims of this research wereto know the optimum
substrate concentration, metalions effect, and value of KM and vmaks of amyoglucosidase. The
result of researches were obtained the optimum activity of amyoglucosidaseat 2% (w/v) of
starch concentration. The activator of amyoglucosidase were Zn2+, Ba2+, Fe3+, and Co2+,
while the inhibitor were Sn2+, Ca2+, Cu2+, Cd2+, Mn2+, Hg2+, and Ni2+. The ions of Hg2+ and
Ni2+were founded stopped of the enzyme activity. The variables of kinetic of amyloglucosidae
were obtained KM and vmaks value were. 4,40 % , 0,49 mU, respectively.

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